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• A mixture of organic acids and thymol protects primary chicken intestinal epithelial cells from Clostridium perfringens infection in vitro

  In this study, the protective effects of a blend of thymol and organic acids against the effects of Clostridium perfringens type A on chicken intestinal epithelial cells were investigated and compared to bacitracin, a widely used antibiotic in poultry production.

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• Phenol-Rich Botanicals Modulate Oxidative Stress and Epithelial Integrity in Intestinal Epithelial Cells

  The aim of this study was to screen the activity of different sources of phenol compounds on intestinal oxidation and barrier integrity in vitro.

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• Assessing intestinal health. In vitro and ex vivo gut barrier models of farm animals: benefits and limitations

  The concept of “gut health" is not well defined, but this concept has begun to play a very important role in the field of animal science. However, a clear definition of GIT health and the means by which to measure it are lacking. In vitro and ex vivo models can facilitate these studies, creating well-controlled and repeatable conditions to understand how to improve animal gut health.

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• A blend of microencapsulated organic acids and botanicals reduces necrotic enteritis via specific signaling pathways in broilers

  The objective was to evaluate the efficacy of AviPlus®P (a microencapsulated blend of 25 % citric and 16.7% sorbic, 1.7% thymol, and 1% vanillin ) to reduce clinical NE and determine the signaling pathways associated with any changes.

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• Isolation, culture, and characterization of chicken intestinal epithelial cells

  Enterocytes exert an absorptive and protective function in the intestine, and they encounter many different challenging factors such as feed, bacteria, and parasites. An intestinal epithelial in vitro model can help to understand how enterocytes are affected by these factors and contribute to the development of strategies against pathogens.

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• The biological effects of microencapsulated organic acids and botanicals induce tissue-specific and dose-dependent changes to the Gallus gallus microbiota

  Microencapsulated organic acids and botanicals have the potential to develop into important tools for the poultry industry. A blend of organic acids and botanicals (AviPlus®P) has previously shown to reduce Salmonella and Campylobacter in chickens; however, changes to the microbiota of the jejunum and ileum have not been evaluated.

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• Organic acids and nature identical compounds can increase the activity of conventional antibiotics against Clostridium perfringens and Enterococcus cecorum in vitro

  OA and NIC were always effective in a dose-dependent manner, even when the antibiotics failed. For several strains, selected combinations of OA or NIC with antibiotics increased the bacterial sensitivity to antibiotics. Therefore, OA and NIC have potential to enhance the efficacy of conventional antibiotics against C. perfringens and E. cecorum.

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• Single components of botanicals and nature-identical compounds as a non-antibiotic strategy to ameliorate health status and improve performance in poultry and pigs

  The use of NIC allows us to properly combine pure compounds, according to the target to achieve. Thus, they represent a promising non-antibiotic tool to allow better intestinal health and general health status, thereby leading to improved growth performance.

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• A microencapsulated feed additive containing organic acids, thymol, and vanillin increases in vitro functional activity of peripheral blood leukocytes from broiler chicks

  4 days of supplementation with a microencapsulated blend made up of citric and sorbic acids, thymol, and vanillin enhanced the in vitro PBL functions of degranulation, oxidative burst, and nitric oxide production compared with the control diet. Collectively, the data suggest feeding broiler chicks a diet supplemented with a microencapsulated blend of citric and sorbic acids, thymol, and vanillin may prime key immune cells making them more functionally efficient and acts as an immune-modulator to boost the inefficient and undeveloped immune system of young chicks.

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• Dietary supplementation with a microencapsulated blend of organic acids and botanicals alters the kinome in the ileum and jejunum of Gallus gallus

  The objective of this project was to determine if feeding a microencapsulated product comprised of a blend of organic acids and botanicals (AviPlus®P) impacts the intestinal kinome of broiler chickens (Gallus gallus).

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• Animal performance passes through gut health

  Intestinal health is one of the most important aspects of animal production. The intestine acts as the main line of defense against pathogens and it is the organ deputed to nutrient absorption. Both functions have pivotal importance for animal health and production.

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• Gut health is the key for animal health and production

  The general concern about animal welfare, especially for laying hens, is continuously increasing in consumers and farmers. This attention leads to spending time and efforts to understand if our birds are stressed or not, through many different indicators both deriving from animal observation (behavior, performance, fearfulness) and laboratory analysis (Alm et al., 2016; Rodenburg et al., 2008).

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• A microencapsulated blend of organic acids and natural identical flavours reduces necrotic enteritis-associated damages in broiler chickens

  Six-hundred ROSS 308 broilers were allocated in 24 pens divided into 3 experimental groups (d0): the control diet (CTR), the control diet added with Galliacid®S at 300 ppm (GAL S), a microencapsulated blend of organic acids and natural identical flavours (EU patent 1391155B1; Vetagro srl, Italy), and the control diet added with a different blend of organic acids (WSB) added at 300 ppm.

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• Role of carvacrol and cinnamaldehyde in broiler cecal fermentations

  Aim of the study was to investigate the role of carvacrol and cinnamladehyde in modulating cecal microflora in broilers in an in vitro fermentation system.

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• Microencapsulation allows slow release of organic acids in the GI tract of broilers

  Aim of the study was to investigate intestinal concentrations of citric and sorbic acid (OA) from a microencapsulated blend and the possible consequences on intestinal fermentations.

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