Botanicals as a zinc oxide alternative to protect intestinal cells from an Escherichia coli F4 infection in vitro by modulation of enterocyte inflammatory response and bacterial virulence
Bonetti et al. (2023). Front. Vet. Sci., 10, 1141561-1147574
Schwein Press
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Thymol as an Adjuvant to Restore Antibiotic Efficacy and Reduce Antimicrobial Resistance and Virulence Gene Expression in Enterotoxigenic Escherichia coli Strains
The aim of this study was to investigate the potential adjuvant effect of thymol to re-establish antibiotic efficacy against highly resistant ETEC field strains. Secondly, we evaluated the modulation of virulence and antibiotic resistance genes. -
Dual Antimicrobial Effect of Medium-Chain Fatty Acids against an Italian Multidrug Resistant Brachyspira hyodysenteriae Strain
In this study we tested four antibiotics (tylosin, lincomycin, doxycycline, and tiamulin) and medium-chain fatty acids (MCFA; hexanoic, octanoic, decanoic, and dodecanoic acid) against an Italian field strain of B. hyodysenteriae and the ATCC 27164 strain as reference. -
Assessing intestinal health. In vitro and ex vivo gut barrier models of farm animals: benefits and limitations
The concept of “gut health" is not well defined, but this concept has begun to play a very important role in the field of animal science. However, a clear definition of GIT health and the means by which to measure it are lacking. In vitro and ex vivo models can facilitate these studies, creating well-controlled and repeatable conditions to understand how to improve animal gut health. -
Development of an in vitro model to study intestinal integrity during an E. coli K88 challenge
Post-weaning diarrhoea is а striking issue of the pig industry and its onset is primarily associated with Escherichia coli К88 (Е. coli). То assess the effectiveness of bioactives in preventing the damages exerted bу the pathogen, the aim of this study was to set-up an in vitro model to mimic an Е. coli challenge on intestinal cells. -
From acidifiers to intestinal health enhancers: how organic acids can improve growth efficiency of pigs
Organic acids have been used for a long time to support pig growth particularly at weaning, and more recently have become the number one alternative to growth promoters to improve the production efficiency of pigs. -
Single components of botanicals and nature-identical compounds as a non-antibiotic strategy to ameliorate health status and improve performance in poultry and pigs
The use of NIC allows us to properly combine pure compounds, according to the target to achieve. Thus, they represent a promising non-antibiotic tool to allow better intestinal health and general health status, thereby leading to improved growth performance. -
Thymol and Carvacrol Downregulate the Expression of Salmonella typhimurium Virulence Genes during an In Vitro Infection on Caco-2 Cells
A real-time PCR study highlighted a significant downregulation of the main virulence genes of Salmonella (hilA, prgH, invA, sipA, sipC, sipD, sopB, sopE2). These findings indicate that thymol and carvacrol could be good candidates for the control of Salmonella typhimurium in pigs. -
Cannabinoid and Cannabinoid-Related Receptors in the Myenteric Plexus of the Porcine Ileum
The endocannabinoid system (ECS) has opened the door to novel therapeutical approaches targeting cancer, pain, anxiety, stress, and inflammatory diseases. The ECS is ubiquitously expressed in almost all members of Animalia, but its precise localization outside the central nervous system is still under investigation. -
Tributyrin differentially regulates inflammatory markers and modulates goblet cells number along the intestinal tract segments of weaning pigs
Butyric acid is widely used in pig production as feed additive to improve growth performance at weaning, based on its intestinal health-promoting action. The aim of this study was to evaluate the intestinal architecture and expression of inflammatory cytokines and tight junctions (TJ) markers in weaning piglets fed with tributyrin, either free or microencapsulated, as dietary source of butyric acid.